Current lab members
(click on the name for a list of publications)
Group leader
Research Associate
I am interested in understanding fundamental cell biological processes, and in particular their connection with development, disease and cancer. My scientific career began with my undergraduate studies in Biochemistry, at the Faculty of Science at Porto University (Portugal). During my PhD I have studied the centromere and this catalysed my interest to understand the regulation of the glue connecting the sister chromatids. Thus, in Claudio Sunkel lab (IBMC, Porto), my first postdoc focused on the identification and functional characterisation of the proteins underlying not only sister chromatid cohesion but also chromosome condensation using Drosophila melanogaster as a model system. Working with Drosophila, I learned to appreciate the importance of studying protein function by phenotypic analysis in whole organisms and of live imaging of cellular events. Therefore, I felt compelled to apply and expand these skills in other model organisms. 
 
My scientific interest in the mitotic machinery lead me study cell division in the early mouse embryo. The first mitotic divisions of early mouse development are acentriolar, making this a fascinating and unique system to explore mechanisms of spindle assembly in absence of centrioles. Pursuing this idea, I have joined David Glover's lab, initially in collaboration with Magdalena Zernicka-Goetz, where my work unveiled previously unknown functions for Plk4, the master regulator of centriole duplication, which I demonstrated to be also required to promote bipolar spindle assembly and microtubule growth in a process that is centriole-independent. Further expanding on the influence of centrosome numbers on the fidelity of cell division, I am currently investigating the mechanisms underlying the role of supernumerary centrosomes on cancer development. I am using a transgenic mouse to induce centrosome amplification by Plk4 over-expression, which results in severe tissue hyperplasia in the absence of the tumour suppressor protein p53. My focus is now to understand the abnormalities resulting from too many centrosomes. I aim to mechanistically address the connection between centrosome number, tumorigenesis or differentiation.  
Research Associate
I got my Master degree in Veterinary Medical Sciences from Mansoura University, Egypt in 2006. After that I obtained my PhD through a channel system scholarship between Egypt (Mansoura University) and Japan (NARO) in 2010. My PhD work was focused on improving the quality of bovine oocytes and pre-implantation embryos by regulating the apoptotic pathway. I got a US-Egypt junior scientist grant to join University of Pennsylvania, USA followed by two years postdoctoral position in Rutgers, the State University of New Jersey. During my stay in USA, my research was focused on proteins responsible for faithful chromosome segregation during oocyte meiosis to understand the underlying causes of aneuploidy. In 2015, I received a very competitive two years Japanese Society of Promotion of Science (JSPS) fellowship where I joined Hokkaido University, Japan. Currently, as an associate professor of Theriogenology, I am on a sabbatical leave from Mansoura University to join Glover’s lab as a Marie Sklodowska-Curie fellow. My research in Glover’s lab is focusing on understanding the differential kinetics and regulation of acentriolar MTOCs in mouse oocytes and early stage embryos and, importantly, how this differential behavior relates to cell polarity and asymmetrical fate determining divisions at these stages.
Laboratory Technician
I joined the Glover lab in 2015 as a gap year after my A-levels. At the end of the year, my role as a technician was extended and I am now studying biology part time with the Open University.
Research Associate
I  am currently doing my second postdoc in the Glover Lab, where I am continuing on work I did during my first postdoc in Kimata Lab. My project focuses on the understanding why the centrosomes of the female germline are eliminated prior to the end of oogenesis in metazoans. Although important for reproduction, centrosome function during oogenesis and the mechanism controlling stability is unclear. I am currently using Drosophila melanogaster as a model, but I am investigating the use of non-model organisms. 
 
I finished my PhD with Isabel Palacios (Department of Zoology, University of Cambridge) in 2015. I studied tissue homeostasis and development using the follicular epithelium of Drosophila melanogaster as a model. The project involved expression-profiling and hit validation/characterisation using CRISPR/cas9 genomic engineering. 
 
Specialties: CRISPR/Cas9 genomic editing in Drosophila, Oogenesis, Cell Cycle, Developmental Biology, Fly genetics. 
Visiting scientist
I'm currently wrestling with confirming and extending the analysis of a large number of mutations that suppress the female sterility of heterozygous polo Scant/+ +.  

Areas of interest: Genetics of mitosis and meiosis  

Genes of interest: any that interact with polo and Scant, singly or in combination  
Research Associate
I am interested in basic questions in cell biology relating to the microtubule cytoskeleton and the cell cycle. 

 I did my PhD in Edinburgh at the Wellcome Trust Centre for Cell Biology in the lab of Prof. Hiroyuki Ohkura, where I studied microtubule regulation and the mechanisms by which microtubule associated proteins such as EB1 and CLIP190 control the behaviour and dynamics of microtubule plus ends. 

 My scientific interests have since led me on a journey from north to south and from the plus to the minus ends of microtubules (more specifically to centrosome research). Currently, I study the heavily regulated process of centriole biogenesis, whereby a pair of “mother” centrioles duplicates in strict coordination with the cell division cycle and in semi-conservative fashion, giving rise to two pairs, each consisting of a “mother” and a “daughter”. On the molecular level this is enacted by a cascade of events, triggered and controlled by the master-regulator Polo-like kinase 4 (Plk4). The activity of Plk4 is tightly controlled and finely-tuned as up-regulation leads centriole number amplification, while conversely, down-regulation of Plk4 blocks centriole biogenesis. 
 
My recent work in Prof. David Glover’s lab has led to the elucidation of how Plk4 is recruited to centrioles and, more importantly, what is the molecular mechanism by which it triggers the entire process.
Visiting PhD Student
I graduated medical biotechnology at Pomeranian Medical University (Szczecin, Poland). Currently, I am PhD student in Mass Spectrometry Laboratory (Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland). At the beginning of my PhD project, I also joined as a visiting PhD student to Prof. David Glover's Group (Department of Genetics, University of Cambridge, UK). In my project I am focused on the structural aspects of proteins and protein complexes which are involved in the centriole duplication cycle. Currently, my main goals are the investigation of dynamics of single centriolar proteins and the identification of the regions of interactions within chosen protein complexes using Hydrogen Deuterium eXchange monitored by Mass Spectrometry (HDX- MS) method. During my PhD studies I had also the opportunity to get familiar with Solid Phase Peptide Synthesis (SPPS) and with various mass spectrometry techniques like protein identification, differential proteomics (label- free method) as well as quantitative methods: global relative proteomic analysis (iTRAQ) and targeted analysis (PRM- parallel reaction monitoring) and I am bringing them with a bit of biochemical and biophysical expertise to Glover's Group research. 
Laboratory Technician
I joined the lab in 2018 after completing my A- Levels at Hills Road Sixth Form. I hope to continue further study at university at the end of my gap year in the Glover Lab
Research Associate
Finishing my studies at Babes-Bolyai University I continued my apprenticeship at the University of Szeged as a PhD student. Here I had the opportunity to master the skills of Drosophila genetics and molecular biology. In 2016, I set in these skills to understand the biology of centrosomes by joining the Glover lab. I am particularly interested in the crosstalk between centrosome and Golgi, as well as in finding additional centrosome components. My work currently focuses on a Golgin protein (we call it Dragon) which apparently has a centrosomal role as well, potentially being the first centrosome component doing “moonlighting”.
Research Associate
In 2012, I got my PhD in Genetics and Molecular Biology at Sapienza – University of Rome (Italy) working on mitotic and meiotic divisions in Drosophila melanogaster. In 2013, funded by a fellowship from FIRC-AIRC (Associazione Italiana per la Ricerca sul Cancro), I moved to Prof. Glover’s lab at Department of Genetics – University of Cambridge (UK) to study centrosomes in Drosophila. Since 2014, I am Research Associate in David Glover’s lab working on centrosomes during Drosophila embryogenesis. Using genetics, live cell imaging, immunofluorescence and biochemistry I am investigating the role of centrosomes during early stages of Drosophila development, in particular I am interested in interactions between centrosomes and membranes and how this is implicated in cell division and ultimately in development.
PhD student
I joined the Glover Lab in October 2016 as a graduate student, after completing my Bachelor’s Degree (Hons.) in Life Sciences at the National University of Singapore, Singapore. As part of my undergraduate research projects and final-year Honours thesis, I focused on characterising the roles of some novel JAK/STAT signalling targeted genes in the maintenance of the Drosophila testis stem cell populations. Moreover, I joined the Sekelsky Lab at the University of North Carolina at Chapel Hill, USA, for a summer research exchange, studying meiotic recombination in Drosophila. My PhD project in the Glover Lab focuses on investigating centriole biogenesis and function. Through concerted studies in C. elegans, Drosophila and cultured human cells, we now have a fundamental understanding of the core components that govern centriole duplication and function. However, it is very likely that our knowledge is far from being complete. Hence, the challenge now is to investigate if there are additional components potentially involved in centriole biogenesis and function.
Research Associate
I am interested in understanding how the regulation and control of cell division are important for the development of the organisms and the maintenance of their integrity in non-pathologic condition. 
 After graduating from the University of Technology of Compiègne (France) with a Biological engineering degree, I pursued advanced studies in Neuropharmacology in Paris VII University/ Ecole Normale Supérieure and joined the lab of Dr Frédéric Saudou at the Institut Curie working on Huntington’s disease. I completed my PhD in Molecular and Cellular Neurobiology focusing on the characterisation of the IGF-1/Akt neuroprotective signaling pathway in Huntington’s disease. I thus gained experience in the analysis of kinases and phosphorylation as well as neuronal intracellular trafficking. Interested in the regulation of cellular processes, I joined the group of Pr David Glover to analyse the role of phosphorylation and the centrosomal/microtubules reorganisation during cell division. 
 My research interests lie in exploring how mitotic or centriolar proteins, by exhibiting several roles at different stages of the cell cycle, can coordinate cell division with early embryonic development and gametogenesis. For this purpose, the fruit fly Drosophila is an ideal model, able to offer rapid access to embryos and gonads for phenotypic analysis as well as low genes redundancy but also shares conserved core mechanisms with humans. 
I am investigating in particular the roles of Greatwall mitotic kinase and its substrate Endos during early embryonic development and how they synergise with other pathways to promote the accurate development of the embryo. 
I am also interested in some centrosomal proteins, which functions have been reported in cell culture but whose contribution in vivo to gametogenesis or development is still under-characterised. 

PhD student
In 2015 I obtained a degree in Molecular Cell Biology from the University of York and in 2016 I completed a Masters by research, as a result of my work in Dr. Dawn Coverley’s lab, also at the University of York. I am now working towards a PhD at the University of Cambridge. My project, funded by a Cambridge Cancer Centre studentship, focuses on understanding the consequences of centrosome amplification in mammalian cells, using mouse pancreatic organoids as a model system. Centrosome amplification has been described as a feature of several types of cancer, including breast cancer and pancreatic cancer, although the specific mechanism linking these two events remains unknown. In order to induce the presence of supernumerary centrosomes, I will use organoids derived from the pancreas of transgenic mice, which allows doxycycline-inducible overexpression of Polo-like-kinase-4 (Plk4), the main driver of centriole duplication. More specifically I will investigate the effect of supernumerary centrosomes on cell proliferation, polarity and differentiation, as well as the link between centrosome amplification and tumorigenesis.